Pancreatic cancer is one of the worst cancer in the world.
If this cancer developed, the symptom is very slightly.
Generally, this cancer has progressed severely, when we feel the backache, poor appetite and so on.
Therefore, we are wanting how to detect the pancreatic cancer at the early stage.
A lot of researchers are searching the superior bio-markers.
This paper indicates that a set of microRNAs, which is short RNA and is well-known to control the cell function, could be available to diagnose the pancreatic cancer.
MicroRNA Alterations of Pancreatic Intraepithelial Neoplasias
Purpose: MicroRNA (miRNA) alterations are likely to contribute to the development of pancreatic cancer and may serve as markers for the early detection of pancreatic neoplasia.Experimental Design: To identify the miRNA alterations that arise during the development of pancreatic cancer, we determined the levels of 735 miRNAs in 34 pancreatic intraepithelial neoplasias (PanIN) and 15 normal pancreatic duct samples isolated by laser capture microdissection using TaqMan miRNA microarrays. Differential expression of selected miRNAs was confirmed by FISH analysis and by quantitative real-time reverse transcription PCR (qRT-PCR) analysis of selected candidate miRNAs in an independent set of PanIN and normal duct samples.
Results: We identified 107 aberrantly expressed miRNAs in different PanIN grades compared with normal pancreatic duct samples and 35 aberrantly expressed miRNAs in PanIN-3 lesions compared with normal pancreatic duct samples. These differentially expressed miRNAs included those that have been previously identified as differentially expressed in pancreatic ductal adenocarcinomas (PDAC; including miR-21, miR-200a/b/c, miR-216a/b, miR-217, miR-146a, miR-155, miR-182, miR-196b, miR-203, miR-222, miR-338-3p, miR-486-3p, etc.) as well as miRNAs not previously described as differentially expressed in these lesions (miR-125b, miR-296-5p, miR-183*, miR-603, miR-625/*, miR-708, etc.). miR-196b was the most selectively differentially expressed miRNA in PanIN-3 lesions.
Conclusions: Many miRNAs undergo aberrant expression in PanIN lesions and are likely to be important in the development of PDAC. The miRNAs, such as miR-196b, whose expression is limited to PanIN-3 lesions or pancreatic cancers could be useful as diagnostic markers. Clin Cancer Res; 18(4); 981–92. ©2011 AACR.
MicroRNA Alterations of Pancreatic Intraepithelial Neoplasias | Clinical Cancer Research
If these miRNAs are detected in blood or serum, the pancreatic cancer treatment will drastically changed and this is the hope of the patients.
